ECOS™ Blue Competent Cells, XL1-Blue
XL1-Blue ECOS™ Blue Competent Cells
Product
XL1-Blue is the most popular strain for blue/white screening. It is also an excellent host strain for routine cloning application using plasmid or lambda vectors. XL1-Blue cells are endonuclease (endA) deficient, which greatly improve the quality of mini-prep DNA, and are recombination (recA) deficient, improving insert stability. The hsdR mutation prevents the cleavage of cloned DNA by the EcoK endonuclease system. The lacIqZΔM15 gene on the F´ episome allows blue-white color screening..
- A strain modified to be ideal for blue/white screening
- Tetracycline resistant
Applications
- Routine cloning application
- Subcloning
- cDNA library
- Blue/white screening
* Technical specifications subject to change without notice.
| # FYE107-10VL (10 preps) | |
|---|---|
| Efficiency >1 x 108 cfu/μg | |
| 100 μl/vial | 10 vials |
| Control Plasmid (pUC19) | 5 μl (10-4 μg/μl) |
| # FYE107-80VL (80 preps) | |
|---|---|
| Efficiency >1 x 108 cfu/μg | |
| 100 μl/vial | 80 vials |
| Control Plasmid (pUC19) | 5 μl (10-4 μg/μl) |
| # FYE108-10VL (10 preps) | |
|---|---|
| Efficiency >5 x 108 cfu/μg | |
| 100 μl/vial | 10 vials |
| Control Plasmid (pUC19) | 5 μl (10-4 μg/μl) |
| # FYE108-80VL (80 preps) | |
|---|---|
| Efficiency >5 x 108 cfu/μg | |
| 100 μl/vial | 80 vials |
| Control Plasmid (pUC19) | 5 μl (10-4 μg/μl) |
| # FYE109-10VL (10 preps) | |
|---|---|
| Efficiency >2 x 109 cfu/μg | |
| 100 μl/vial | 10 vials |
| Control Plasmid (pUC19) | 5 μl (10-4 μg/μl) |
| # FYE109-80VL (80 preps) | |
|---|---|
| Efficiency >2 x 109 cfu/μg | |
| 100 μl/vial | 80 vials |
| Control Plasmid (pUC19) | 5 μl (10-4 μg/μl) |
| # FYE107-96WL (96 preps) | |
|---|---|
| Efficiency >1 x 108 cfu/μg | |
| 50 μl/well | 96-wells |
| # FYE108-96WL (96 preps) | |
|---|---|
| Efficiency >5 x 108 cfu/μg | |
| 50 μl/well | 96-wells |
| # FYE109-96WL (96 preps) | |
|---|---|
| Efficiency >1 x 109 cfu/μg | |
| 50 μl/well | 96-wells |
Genotype recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [F’proABlacIqZDM15 Tn10 (TetR)]
| Cat. No. | Product Description |
|---|---|
| # FYE107-10VL | 10 preps |
| # FYE107-80VL | 80 preps |
| # FYE108-10VL | 10 preps |
| # FYE108-80VL | 80 preps |
| # FYE109-10VL | 10 preps |
| # FYE109-80VL | 80 preps |
| # FYE107-96WL | 96 preps |
| # FYE108-96WL | 96 preps |
| # FYE109-96WL | 96 preps |
