ECOS™ Competent Cells
The fastest transforming cells worldwide- 1 minute transformationWith “ECOS™” standing for “E. Coli One-Step”, and its reverse “SOCE” standing for “SOC Eliminated”, the name itself speaks for the advantages.
1 minute, 1 step high-efficiency transformation and no SOC recovery step required, truly a design to save your time.
| Competent Cell Types | ECOS™ Competent Cells | Traditional Chemically Competent Cells | Electro-Competent Cells |
|---|---|---|---|
| Time | 1 ~ 6 minutes | 1.5 ~ 2 hours | 1.5 ~ 2.5 hours |
| Protocol |
|
|
|
| Authentic Representation | High | Require recovery step | Require recovery step |
| Transformation Efficiency | 107 ~ 1010 cfu/µg pUC19 | 106 ~ 109 cfu/µg pUC19 | ~ 1010 cfu/µg pUC19 |
| Cost | Low | High | High |
Patented Technology
Yeastern Biotech Co., Ltd. owns the patent of ECOS™ technology (protocol) exclusively in USA, UK, Taiwan, and France (Patent No. US 6,864,088; GB 2383582; I 229696; FR 2832727, etc.). Under the protection of this patent, Yeastern Biotech is the only company that has the right to use the protocol with the claims that transformation with our ECOS™ chemically competent cells can be finished within one minute and SOC is not needed. If anyone intends to sell competent cells that use ECOS™ protocol or its similar version, please contact our headquarters in Taiwan for licensing.
Quality Control
Each lot of competent cells has to pass three quality control tests before shipping:- Efficiency test: each batch of ECOS™ should meet the claimed transformation efficiency at the time of production using Protocol 4 (Page 3-3) and supercoiled pUC19 DNA.
- Contamination test: competent cells were plated directly on ampicillin plates without being transformed. To pass the test, no colonies should be seen after overnight incubation.
- A-complementation test: this test is performed for all ECOS™ competent cells except for ECOS™ 21(DE3) and ECOS™ 2163. To pass the test, the ratio of white colonies over the total colonies should be less than 3%
