ECOS™ 9-5 Competent Cells, JM109
Product
JM109, a K strain bacterium, carries mutations in recA and endA, leading to minimal recombination and improved quality of isolated plasmid DNA. In addition, the cells carry the F’ episome which allows blue/white screening for recombinant DNA and the production of ssDNA. Thus, it has been a popular strain for routine subcloning. The cells can also be utilized for single-stranded DNA rescue when M13 or phagemid systems are used simultaneously. The JM109 strain is sensitive to all common antibiotics.
This product can become a powerful cloning tool when it is used in combination with Yeastern’s rapid ligation kit (#FYC003-100R). The ligation can be finished within 5 minutes and followed by 1-minute transformation. The fast growth rate of JM109 allows colonies to show up within 8 hours. Try this strain when you are in urgent.
- Rapid growing strain
- Carries F’ episome
Applications
- Preparation of ssDNA
- Construction of gene library
- Subcloning
* Technical specifications subject to change without notice.
| # FYE707-10VL (10 preps) | |
|---|---|
| Efficiency >5 x 107 cfu/μg | |
| 100 μl/vial | 10 vials |
| Control Plasmid (pUC19) | 5 μl (10-4 μg/μl) |
| # FYE707-80VL (80 preps) | |
|---|---|
| Efficiency >5 x 107 cfu/μg | |
| 100 μl/vial | 80 vials |
| Control Plasmid (pUC19) | 5 μl (10-4 μg/μl) |
| # FYE707-50-20VL (20 preps) | |
|---|---|
| Efficiency >5 x 107 cfu/μg | |
| 50 μl/vial | 20 vials |
| Control Plasmid (pUC19) | 5 μl (10-4 μg/μl) |
| # FYE708-10VL (10 preps) | |
|---|---|
| Efficiency >1 x 108 cfu/μg | |
| 100 μl/vial | 10 vials |
| Control Plasmid (pUC19) | 5 μl (10-4 μg/μl) |
| # FYE708-80VL (80 preps) | |
|---|---|
| Efficiency >1 x 108 cfu/μg | |
| 100 μl/vial | 80 vials |
| Control Plasmid (pUC19) | 5 μl (10-4 μg/μl) |
| # FYE709-10VL (10 preps) | |
|---|---|
| Efficiency >5 x 108 cfu/μg | |
| 100 μl/vial | 10 vials |
| Control Plasmid (pUC19) | 5 μl (10-4 μg/μl) |
| # FYE709-80VL (80 preps) | |
|---|---|
| Efficiency >5 x 108 cfu/μg | |
| 100 μl/vial | 80 vials |
| Control Plasmid (pUC19) | 5 μl (10-4 μg/μl) |
| # FYE707-96WL (96 preps) | |
|---|---|
| Efficiency >5 x 107 cfu/μg | |
| 50 μl/well | 96-wells |
| # FYE708-96WL (96 preps) | |
|---|---|
| Efficiency >1 x 108 cfu/μg | |
| 50 μl/well | 96-wells |
| # FYE709-96WL (96 preps) | |
|---|---|
| Efficiency >5 x 108 cfu/μg | |
| 50 μl/well | 96-wells |
Genotype e14–(McrA–) recA1 endA1 gyrA96 thi-1 hsdR17(rK– mK+) supE44 relA1 D(lac–proAB) [F’ traD36 proAB lacIqZDM15]
| Cat. No. | Product Description |
|---|---|
| # FYE707-10VL | 10 preps |
| # FYE707-80VL | 80 preps |
| # FYE707-50-20VL | 20 preps |
| # FYE708-10VL | 10 preps |
| # FYE708-80VL | 80 preps |
| # FYE709-10VL | 10 preps |
| # FYE709-80VL | 80 preps |
| # FYE707-96WL | 96 preps |
| # FYE708-96WL | 96 preps |
| # FYE709-96WL | 96 preps |
