ECOS™ 21 Competent Cells, BL21(DE3)
BL21(DE3) ECOS™ 21 Competent Cells
Product
This strain provides high levels of protein expression. This strain carries the lambda DE3 lysogen, which expresses T7 RNA polymerase from the lacUV5 promoter by isopropyl-1-thio-ß-D-galactopyranoside (IPTG) induction. The transformation efficiency of BL-21 is usually low, so the 6 minute/ heat shock cold plating protocol is recommended if high efficiency is desired.
Genotype
F– ompT gal dcm lon hsdSB(rB– mB–) λ(DE3 [lacI lacUV5-T7 gene 1 ind1 sam7 nin5]).
- lack of Lon and Omp proteases allows high-level protein expression and accumulation
- Easy gene induction
Applications
- IPTG induced gene expression in E. coli
- Ideal for high-level expression of non-toxic recombinant protein expression using T7-based expression vectors
- Subcloning
* All images are for reference only, actual products might differ from the pictures above.
* Technical specifications subject to change without notice.
| # FYE207-5VL (5 preps) | |
|---|---|
| Efficiency >2 x 107 cfu/μg | |
| 100 μl/vial | 5 vials |
| Control Plasmid (pUC19) | 5 μl (10-4 μg/μl) |
| # FYE207-40VL (40 preps) | |
|---|---|
| Efficiency >2 x 107 cfu/μg | |
| 100 μl/vial | 40 vials |
| Control Plasmid (pUC19) | 5 μl (10-4 μg/μl) |
| # FYE207-96WL (96 preps) | |
|---|---|
| Efficiency >2 x 107 cfu/μg | |
| 50 μl/well | 96wells |
| Cat. No. | Product Description |
|---|---|
| # FYE207-5VL | (5 preps) |
| # FYE207-40VL | (40 preps) |
| # FYE207-96WL | (96 preps) |
