Major Red Plus stain, MRP-500

Product

Major Red Plus stain is a non-mutagenic fluorescent dye that enables instant visualization of DNA/RNA bands in agarose gels under blue light or UV illumination. Formulated in major science’s 6X DNA Loading Buffer, it is used to prepare DNA markers and samples for electrophoresis on agarose or polyacrylamide gels. Major Red Plus Stain offers high sensitivity for detecting double-stranded DNA (dsDNA). The loading buffer contains tracking dyes to facilitate monitoring of DNA migration during electrophoresis. This product provides a safe, non-hazardous alternative to ethidium bromide, making it ideal for laboratories seeking environmentally friendly DNA staining solutions.

Protocol: Gel Casting Method (In-Gel Staining)
1. Add Major Red Plus to molten agarose before casting the gel.
2. Example: Add 2μL of Major Red Plus (10,000× concentrate) to 20mL of agarose solution.
3. Perform electrophoresis as usual.
4. Observation after electrophoresis:
    a. Use a UV light or blue light transilluminator for visual observation, and always apply the appropriate and safety filter.
    b. For gel documentation, use UV light or blue light as the source, and always apply the appropriate and safety filter.

Notes:
    • This method is cost-effective: 500μL of Major Red Plus can prepare ~250gels (20mL per each gel).
    • Due to its thermal stability, Major Red Plus can be added directly to hot agarose without cooling. Mix thoroughly by swirling, vortexing, 
      or inverting. Alternatively, add dye to agarose powder and buffer before heating.
    • Dye-containing agarose can be prepared in bulk and stored until use.
    • Not suitable for precast polyacrylamide gels.

DNA Dye	Blue/Green LED	Blue LED	UV-Light	In-Gel	Poststaining	Direct
Major Red Plus stain