Product Name : SIP Fermentation System (Pilot Scale), FS-10L/ FS-30L / FS-50L
Product Description

(FS-10L / FS-30L / FS-50L) Pilot bioreactor(In situ Sterilizable)

Product Introduction

The 10L / 30L / 50L SIP Pilot Scale Fermentation Systems allow you to run small volume of fermentation experiment with ease. These systems are perfectly suitable for small scaling up in the academic lab. The easy programming and operation on our Winpact software put your operation procedure in your peace of mind. System comes with rolling wheels for mobility. All materials are made from the highest standard to ensure optimization of your fermentation process. A variety of optional devices are also available for selection to maintain your optimum performance.

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A.Harvest valve:  sterilizable, diaphragm-type tank bottom valve for sanitary post-process harvest

B.Sampling valve: steam-sterilizable valve permits repeatedly sanitary sample collection

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B1.Motor - Manual or automatic control of constant agitation speed.

B2.Stainless Steel - Vessel Special designed heating jacket provides better heating efficiency

B3.Peristaltic Pump - Uses four Watson Marlow built-in peristaltic pumps for all your feeding needs

B4.Control Station - Large screen and graphical user interface

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Major Science SIP Fermentation Systems

•  Wide range of vessel selection, from 10L to 50L working volume

•  Colorful interface at 10.4" or above

•  Fully automated process with remote monitoring

•  15-step automatic program setting

•  Orbital welding ensures minimal residue buildup

•  Highest grade construction with 316L stainless steel

•  Jacket design provides astounding temperature control

•  Exhaust pressure relief valve for maximum safety precaution

•  Multiple safety design integration for peace of mind operation

•  Remote monitoring & controlling software free from purchase

•  Password protection for multiple users with special requirements

•  Various optional devices for process optimization and needs

•  Ethernet communication with Winpact SCADA software, and IP address

 

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Four-staged DO cascade

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15-Step programmable PID control

Immediate visualization on operation overview

Easy and intuitive operation for manual and sequence control

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One-Touch automatic sterilization for

vessel and system tubing

Online system calibration with

system feedback

System expansion with various

optional devices

*Technical specification subject to change without notice.
*All images are for reference only, actual products might differ from the pictures above.

Capacity

10 L

30 L

50 L

Total volume

15 L

42 L

67 L

Working volume

10 L

30 L

50 L

Vessel and jacket maximum working pressure

3 bar (43.5 psig) / 4 bar (58 psig)

Type

Double layered fully enclosed capsule-type tank

Material

Direct contact to medium - 316L stainless steel; all others - 304 stainless steel

Surface finish

Interior polish  25 Ra/in (0.6 μm) Mechanical polishing; Electropolish optional
Exterior polish 
 32 Ra/in (0.8 μm) Mechanical polishing; Electropolish optional

Ports

Ports designed according to user requirements

Piping and valve materials

Parts that directly contact with the product/medium uses 316L stainless steel (≤25 Ra/in) internal polished tubing (BPE standard) :
A.) Internal EP polished diaphragm type pneumatic valve and manual valve (BPE standard)
B.) Tubing all welded with orbital welding
C.) Vessel bottom drain uses a diaphragm valve, to minimize dead volume
D.) Piping designed for ease of transfer to scale up (can be used as a seed fermentation system) or downstream process

Parts that do not directly contact product/medium
A.)
Constructed with 304 stainless steel

Controller

10.4” color industrial touch screen

* User-friendly, graphical control interface
* Includes secure user accounts, with different levels of access
* Modularized and standardized design (Module Skid): ergonomically designed according to height, ease of vessel clean
   up,  and ease of extraction in relation to vessel bottom valve
* Includes maintenance page with system diagnostics
* All programmed setting values are automatically stored into the built-in memory; the settings will not be lost in case of
   power outage/interruption. When power is restored, the interrupted process will automatically resumed

Setting

* Automated sterilization process
* Automated fermentation program

DO

* Control range: 0-100%, adjustable

* Software electrode calibration, with automatic temperature compensation function
* Includes one set of side-inserted stainless steel autoclavable DO electrode

* DO Stat features with intelligent feeding

pH

* PID control with adjustable deadband
* Control range 2.00 to 12.00 pH, ±0.01
* Calibration function with automatic temperature compensation function
* Includes one set of side-inserted, autoclavable pH probe with stainless steel housing

* pH Stat features with intelligent feeding

Pump

* Built-in peristaltic pumps

* Each feeding pump can run adjustable 15-step program
* Each pump can be adjusted for speed, forward and backward direction, and manual or automatic mode
* Each of the four peristaltic pumps can be designated for different functions: acid pump, base pump, antifoam pump, or 
   substrate feeding pump
* Optional fifth and six peristaltic pump available

Temperature

* Vessel temperature is measured with a side-inserted PT-100 temperature probe and maintained using PID control.
* Control range: 0-130°C, ±0.1°C. Operational range up to 0-60°C

Agitation

* Manual or automatic control of agitation speed
* 15-step program to change speed, or use DO cascade control

Air supply and exhaust

Gas supply and dehumidifer: uses in-house air compressor or air dehumidifier
* Includes re-useable, autoclavable 0.2μm air filter for gas inlet
* Gas Inlet (air)
    Includes mass flow controller:
    2 vvm maximum according to the vessel capacity
* Gas Inlet (oxygen)
   Includes pure oxygen rotameter (manual flow control):
   1 vvm maximum according to the vessel capacity
* Includes oxygen gas solenoid valve, with automatic pulsed time control

Air outlet / Exhaust
* Exhaust port with stainless steel condenser
* Includes re-useable, autoclavable 0.2μm air filter
* Includes automated adjustable gas outlet valve to adjust vessel back pressure
* Can control manually or automatically via software

 

Utility Requirement

Capacity

10 L

30 L

50 L

Power

Three phase 220V or 380V (note: can be customized to local standard)

Air

At least 6 bar

30 L/min flow rate

90 L/min flow rate

150 L/min flow rate

Dehumidified

Oil-free

Peripheral factory water supply

Cooling water (tap water, at least 15oC below working temperature, must be soft water) ;
Pressure at least 2 bar

Process water

RO Water

Plant steam

≥ 2 bar; dry steam with no entrained condensate

Process steam

≥ 2 bar; dry steam with no entrained condensate

Drain

In situ drain; ≥ 1"

*Customization on the SIP Fermentation system available upon request. Please contact your regional manager for evaluation request.

Optional Accessory Items

-Electropolish (EP) of Vessel Tank

Electropolish of the vessel tank is offered as a higher sanitary grade surface finish. EP surface finish is an addition to the standard mechanical polish (MP) which provides a smoother surface area to minimize residual residue.

*Note that this option MUST be requested at your initial inquiry, later-on additions after completion of construction is not possible

 

-Transfer Piping

Transfer piping of your resulting product/medium between vessels is offered for convenience of operation.

Automated transfer using pressure in addition to directional control with valve regulations offers fast and easy operation.

 

-ORP Probe

The ORP probe measures the oxidation-reduction potential of the fermentation media, which is a crucial indicator of anaerobic 

conditions/reactions. This low maintenance and sterilizable probe is designed to withstand repeated experiment.

 

-Turbidity Probe

Turbidity probes are used to measure the total cell density of fermentation media, which provide you an indication for metabolic reactions and relevant information for cell and bacteria cultures.

 

-Load Cell

The load cell provides weighing capability to your fermentation process. Weighing the substrate feeding can indicate how much substrate was added during the fermentation process, which allows the user to provide more accurate control accordingly to what the process needs.

 

-Cell Density Monitor

Our special implemented online cell density device allows you to obtain direct and continuous information for cell growth analysis and cell density as they are crucial for eukaryotic cells, yeast and bacteria fermentation. Having trouble determining cell growth rate? Our online cell density is the key to your solution.

 

-Oxygen Mass Flow Controller

Maintain optimal control over culture DO level by installing this optional mass flow controller. The mass flow controller can accurately adjust the flow rate of incoming oxygen and is resistant to fluctuations in gas pressure, ensuring precise control and repeatibility of experimental conditions

A.) Cascade control scheme

B.) Integrated into controller for simple and automated operation

 

-Gas Mixing Station

The gas mixing station allows the user to optimize cell growth conditions by independently supplying up to four gasses to the fermentation process. Parameters such as dissolved oxygen and pH can be controlled by adjusting the gas composition supplied to the system. Four manually adjusted flow meters control the flow rate of each gas, while the 4 solenoid valves automatically open or close in response to the culture conditions. The Gas Mixing Station can be operated in either manual or automatic modes.

 

-CO2 / O2 Off-Gas Analyzer

The CO2 / O2 off-gas analyzer provides real-time measurement of carbon dioxide and oxygen concentration of the bioreactor exhaust gas. The CO2 concentration is determined using a self-calibrating non-dispersion infrared sensor, while an electrochemical sensor monitors the oxygen concentration. Using this information, the user can continuously monitor metabolism and analyze cell growth parameters.

 

-Headplate Lift

For SIP vessel headplate lifting, an device for headplate lifting/removal. Chain-hoist system (manual) for 30 L / 50L.

Catalogue_W04_SIP Fermentation

Brochure-012-SIP

Winpact_Cata_04_SIP