Product Name : Major Cycler, CYCLER-25
Product Introduction

Major Cycler is an automated instrument designed for application in hot start thermal cycling reactions with additional reverse transcription (RT) program function, it is simple and convenient to run one-step RT by combining the RT program with a stored amplification reaction. Another feature of Major Cycler is the flexible program editing, which allows researchers to set up an amplification program containing different temperature ramping conditions within individual loops. Major Cycler also offers the flexibility to create an advanced amplification program which could increase/decrease temperatures and hold time for an assigned step from cycle to cycle. The small size and competitive pricing make it suitable for a personal instrument ownership.

MS03_Thermal Cycler CYCLER-25 * Marketing video

• Microprocessor control with digital performance

• Wide temperature control range and great temperature controlling performance

• LCD display

• Self pressure adjusting heating lid

• User friendly and powerful program performance

• RT program

• Link up to 5 loops for one program

• Up to 9 steps for each loop

• Up to 99 cycles for each loop


Fig.1 Amplification efficiency comparison between Brand A cycler and Major Cycler.
M.DNA marker
A1-A6 DNA fragment amplified by Brand A cycler
M1-AM6 DNA fragment amplified by Major Science


Fig.2 Reverse Transcription and amplification of 900 bp Japanese Encephalitis Virus (JEV) envelope protein fragment from JEV RNA using Major Cycler RT and amplification program
M. DNA marker
1. 0.1 μg RNA template
2. 0.5 μg RNA template
3. 1.0 μg RNA template


 Cat. No.


Sample capacity

 25(5x5)x0.2ml tube

Temperature control range

 4°C to 110°C

Lid temperature control  range

 ambient + 5°C to 110°C

Block homogeneity

 20°C to 72°C ≤ ± 0.3°C; 95°C: ≤±0.4°C

Control accuracy

 ± 0.2°C

Heating rate

 approx. 3°C / s

Cooling rate

 approx. 2°C / s


 2.6" LCD


 Reverse transcription program

 Link program: multi-loop available; up to 5

 Step per Loop: up to 9

 Cycle number: up to 99

 Program storage: up to 100

Increment and decrement temperataure per step on each cycle

Increment and decrement time per step on each cycle


Rated voltage

 110V / 220V selectable

Dimension (WxLxH)

 (WxLxH) 7.87"x12.62"x7.48" (200x320.5x190mm)


Approx. 18.9lb (8.6kg)

Cat. No.

Product Description


Major Cycler, 25 wells